cell culture human normal prostate epithelial cells rwpe  (ATCC)

Journal: Toxicology and applied pharmacology

Article Title: Role of the PI3K/Akt pathway in cadmium induced malignant transformation of normal prostate epithelial cells.

doi: 10.1016/j.taap.2020.115308

Figure Lengend Snippet: Cell phenotype and tumor formation in nude mice. Parental (RWPE1/PWR1E) and cadmium-exposed (Cd-RWPE1/Cd-PWR1E) cells under the reflected light microscope (A). Sketch showing injection scheme (B). Tumor formation by Cd-RWPE1 cells (C-D). Batch matched parental RWPE1 cells did not result in any tumors (D). Tumor volume over the course of experiment (D). There were four replicates for each cell line. Error bars ±SE.

Article Snippet: Human prostate epithelial cell lines and their culture Human normal prostate epithelial cell lines RWPE1 and PWR1E were purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Light Microscopy, Injection

Journal: Toxicology and applied pharmacology

Article Title: Role of the PI3K/Akt pathway in cadmium induced malignant transformation of normal prostate epithelial cells.

doi: 10.1016/j.taap.2020.115308

Figure Lengend Snippet: Wound healing assay. Percent artificial wound closure after 24 hours (A; G). Representative pictures of the artificial wound at 0 and 24 hours of cell culture (B; H). Error bar ±SD. Chemotactic cell migration and invasion assay. Average percent migration (C; I) and invasion (E; K) in Cd-exposed (Cd-RWPE1 and Cd-PWR1E) cells compared to their normal parental (RWPE1 and PWR1E) controls. Data are represented as the mean ±SD from at least three replicates. Representative pictures of migration (D; J) and invasion (F; L) in both cell lines.

Article Snippet: Human prostate epithelial cell lines and their culture Human normal prostate epithelial cell lines RWPE1 and PWR1E were purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Wound Healing Assay, Cell Culture, Migration, Invasion Assay

Journal: Toxicology and applied pharmacology

Article Title: Role of the PI3K/Akt pathway in cadmium induced malignant transformation of normal prostate epithelial cells.

doi: 10.1016/j.taap.2020.115308

Figure Lengend Snippet: Differential expression by RT2 profiler array, real-time PCR and immunoblot analyses of the PI3K/Akt signaling pathway. Scatter plot representing the array results. Upregulated genes are indicated by red points and downregulated genes are in green (A). Heat Map shows the fold changes in expression of 84 PI3K/Akt pathway genes in Cd-RWPE1 vs. RWPE1 cells (B). The complete gene names, location on array and fold changes are indicated in Supplemental Table 1. TaqMan gene expression (C-I) and immunoblot (J) analyses of selected PI3K/Akt pathway genes. Graphical representation of the relative fold upregulation in protein levels based on the average intensity of the target proteins in all replicates and normalized to their endogenous controls (K). Error bars ±SD. Experiments were performed three times and each time there were three replicates in TaqMan assays. For RT2 profiler array RNA from three biological replicates was pooled together for the cDNA synthesis for all four cell lines.

Article Snippet: Human prostate epithelial cell lines and their culture Human normal prostate epithelial cell lines RWPE1 and PWR1E were purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Quantitative Proteomics, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Gene Expression, cDNA Synthesis

Journal: Toxicology and applied pharmacology

Article Title: Role of the PI3K/Akt pathway in cadmium induced malignant transformation of normal prostate epithelial cells.

doi: 10.1016/j.taap.2020.115308

Figure Lengend Snippet: Significantly up- (> 2 fold) and down-regulated ( <0.5) genes in Cd exposed (Cd-RWPE1; Cd-PWR1E) compared to parental normal (RWPE1; PWR1E) cells.

Article Snippet: Human prostate epithelial cell lines and their culture Human normal prostate epithelial cell lines RWPE1 and PWR1E were purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Techniques:

Journal: Toxicology and applied pharmacology

Article Title: Role of the PI3K/Akt pathway in cadmium induced malignant transformation of normal prostate epithelial cells.

doi: 10.1016/j.taap.2020.115308

Figure Lengend Snippet: Cd-RWPE1 is denoted as “1” and parental normal RWPE1 as “0” in the plots. Five overlapping pathways are enriched in Cd-RWPE1 vs. Parental RWPE1 as control. These pathways are KEGG_Prostate cancer Pathway (A). KEGG_Apoptosis (B). KEGG_MAPK (C). KEGG_Pathways in cancer (D). KEGG_ERBB (E) pathways. Same pathways were enriched in Cd-PWR1E vs. normal parental PWR1E cells (Supplemental Figures 1 and 2).

Article Snippet: Human prostate epithelial cell lines and their culture Human normal prostate epithelial cell lines RWPE1 and PWR1E were purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Control

Journal: Toxicology and applied pharmacology

Article Title: Activation of the Erk/MAPK signaling pathway is a driver for cadmium induced prostate cancer.

doi: 10.1016/j.taap.2020.115102

Figure Lengend Snippet: PCR array analysis of the Erk/MAPK signaling pathway. Scatter plot comparison of normalized gene expression between RWPE1 and Cd-RWPE1 cells. The central line indicates unchanged gene expression. Data points in red are the upregulated genes and in green are downregulated genes of the Erk/MAPK pathway (A). Heat Map visualization of the fold changes in expression in 84 genes of the Erk/MAPK pathway in Cd-RWPE1 compared to RWPE1 cells (B). The gene names and fold regulation along with the location on array are indicated in Supplemental Table 2. Quantitative real-time PCR analysis of selected Erk/MAPK pathway genes utilizing TaqMan gene expression assays in Cd-RWPE1 vs. parental normal RWPE1 (C) and Cd-PWR1E vs. parental normal PWR1E (D). Immunofluorescence assay (E). Error bars ±SD.

Article Snippet: Cell lines and culture Human normal prostate epithelial cells RWPE1 and PWR1E and prostate cancer cell line DU 145 were obtained from the American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Comparison, Gene Expression, Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence

Journal: Toxicology and applied pharmacology

Article Title: Activation of the Erk/MAPK signaling pathway is a driver for cadmium induced prostate cancer.

doi: 10.1016/j.taap.2020.115102

Figure Lengend Snippet: Functional implication of Cd exposure in normal prostate epithelial cells. Cell proliferation assay shows increased proliferation and colony formation in Cd-RWPE1 compared to parental RWPE1 cells (A; B-C) and in Cd-PWR1E vs. parental normal PWR1E (D; E-F). FACS analysis for cell cycle distribution showed an increase in the S-phase population (C-D) and a decrease in the apoptotic cell fraction (E-F) in Cd-RWPE1 compared to parental RWPE1 cells. Similar effects were observed in Cd-PWR1E vs. normal parental PWR1E (K-L). Error bars ±SD.

Article Snippet: Cell lines and culture Human normal prostate epithelial cells RWPE1 and PWR1E and prostate cancer cell line DU 145 were obtained from the American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Functional Assay, Proliferation Assay

Journal: Toxicology and applied pharmacology

Article Title: Activation of the Erk/MAPK signaling pathway is a driver for cadmium induced prostate cancer.

doi: 10.1016/j.taap.2020.115102

Figure Lengend Snippet: Gene Set Enrichment Analysis (GSEA) of the Erk/MAPK signaling pathway enriched in Cd-RWPE1 (denoted as “1” in the plot) compared to RWPE1 (denoted as “0” in the plot) as control. Differential expression of genes (A) correlated with the enrichment plot (B) of MAPK signaling pathway genes. Red-overexpression, Blue-lower expression. The gene lists counterpart to each gradient red and blue bar that is located at the middle of Enrichment plot and indicate the genes assigned in the plot.

Article Snippet: Cell lines and culture Human normal prostate epithelial cells RWPE1 and PWR1E and prostate cancer cell line DU 145 were obtained from the American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Control, Quantitative Proteomics, Over Expression, Expressing

Journal: Toxicology and applied pharmacology

Article Title: Activation of the Erk/MAPK signaling pathway is a driver for cadmium induced prostate cancer.

doi: 10.1016/j.taap.2020.115102

Figure Lengend Snippet: Gene Set Enrichment Analysis of pathways enriched in Cd-RWPE1 compared to RWPE1 as control. KEGG_Pathways in cancer (A). KEGG_Cell Cycle (B). KEGG_ERBB (C). KEGG_Prostate Cancer (D). KEGG_VEGF (E). Enrichment plots are shown in Supplemental Figure 2. Red-overexpression, Blue-lower expression. The gene lists counterpart to each gradient red and blue bar that is located at the middle of Enrichment plot in Supplemental Figure 2A–E and indicate the genes were assigned in the plot.

Article Snippet: Cell lines and culture Human normal prostate epithelial cells RWPE1 and PWR1E and prostate cancer cell line DU 145 were obtained from the American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Control, Over Expression, Expressing